Dissertation > Excellent graduate degree dissertation topics show

The Relationship between NF-κB and Epithelial-to-mesenchymal Transition of Radiation-induced Human Esophageal Carcinoma Cells

Author: YangZuo
Tutor: XueXiaoYing
School: Hebei Medical University
Course: Oncology
Keywords: esophageal cancer radiotherapy NF-κB E-cadherin Vimentin radiation resistance
CLC: R735.1
Type: Master's thesis
Year: 2014
Downloads: 5
Quote: 0
Read: Download Dissertation

Abstract


Objective:-To=Explore=the=relationship=between=NF-κB=and=epithelial-to-mesenchymal transition of human esophageal carcinoma cells andprovide theoretical foundation for formulating reasonable and effectiveindividualized treatment plan of esophageal carcinoma.Methods: Using the method of gradient irradiation dose, to make thehuman esophageal carcinoma epithelial cell Eca109to Eca109R60by thecumulative dose of60Gy. Changes in cell morphology were observed byoptical microscope. Meanwhile, real-time PCR and immunocytochemistrywere used to detect the mRNA and protein level of NF-κB, E-cadherin andVimentin in esophageal epithelial cell lines Eca109and Eca109R60.Result:1Observed under a microscope, compared with esophageal carcinomaepithelium Eca109, Eca109R60cell showed more irregular, elongatedspindle-shaped, loss of polarity, and more loose in cell adhesion.2The relationship between mRNA of NF-κB, E-cadherin and Vimentin.In non-irradiated Eca109cells, E-cad mRNA expression wassignificantly higher than that in Eca109R60cells(P=0.003). The differencewas statistically significant(P<0.01). In non-irradiated cells Eca109, VimmRNA expression is significantly lower than that in irradiated cellsEca109R60(P=0.004). The difference is statistically significan(tP<0.01). Innon-irradiated cells Eca109, NF-κB mRNA expression is lower than that inirradiated cells Eca109R60(P=0.004). The difference is statistically significant(P<0.01). Relationship with each other after radiotherapy, NF-κB andVimentin, E-cadherin mRNA expression as follows. Linear relationshipbetween NF-κB and Vimentin is not statistically significant(P=0.747).However, their have a positive correlation in some extent. Linear relationshipbetween NF-κB and E-cadherin is also not statistically significant(P=0.061).It means NF-κB and E-cadherin expression have a negative correlation.3The relationship between the protein expression of NF-κB, E-cadherinand Vimentin. Results of immunocytochemistry showed Expression of theepithelial cell marker E-cadherin was mainly located in the cytoplasm,brownish yellow in esophageal carcinoma cells. The expression of E-cadherinprotein in cells Eca109was stronger than that in Eca109R60by microscopicobservation. Expression of the mesenchymal cell marker Vimentin proteinwas mainly located in the cytoplasm, brownish yellow in esophagealcarcinoma cells. The expression of Vimentin protein in cells Eca109waslower than that in Eca109R60by microscopic observation. The mostcommon subunits of NF-κB was p65/p65. NF-κB p65protein was expressedin the cytoplasm and nucleus in esophageal cancer cells. But the NF-κB p65protein was main expression in the nucleus when it was activated,brownish-yellow granules. The expression of NF-κB p65protein in cellsEca109was weaker than that in Eca109R60by microscopic observation.Conclusion:1After irradiation, the esophageal carcinoma cell Eca109R60showedradiation resistant.2The esophageal carcinoma radiation-resistant cell lines Eca109R60occured epithelial-to-mesenchymal transition,the marker downregulation ofE-cad, Vim upregulated.3In some extent, the expression of E-cad and NF-κB are negativelycorrelated. In a other hand, the expression of Vim and NF-κB are positivelycorrelated. Suggesting that NF-κB might be involved in esophageal carcinomacells occur epithelial-to-mesenchymal transition process, affecting theradiosensitivity of esophageal carcinoma.

Related Dissertations

  1. Study the Protective Value of Spect Lung Perfusion to Lung Function in Esophageal Cancer Patients Treated with Radiotherapy,R735.1
  2. The Clinical Study of Cone-beam CT Combining with Active Breathing Control System on Lung Cancer Radiotherapy,R734.2
  3. Research on BT Toxin Oligomer Formation Induced by Lipid Raft and the Immunodetection Explore of Cadherin,S476.1
  4. Fitness Costs of a Disrupted Cadherin Allele Conferring Cry1Ac Resistance of Helicoverpa Armigera,S435.622.3
  5. The Effects of PCV2 on NF-κB Signal in Piglet’s Lymphocytes in Vitro,S858.28
  6. Nuclear Dot Protein NDP52 Bind to Tumor Necrosis Factor Receptor Associated Factor 6 and Their Clinical Significance of Research,R363
  7. Effective of the Interaction between NDP52 and TRAF6 and TRAFs on NF-κB Signal Pathway,R363
  8. The Effect and Mechanism of p38MAPK Inhibitor CBS3830 on Intimal Hyperplasia in Autogenous Vein Grafts of Diabetic Rats,R587.1
  9. Effects of Partial Hepatic Ischemia/Reperfusion Injury on Postoperative Cognitive Function in Mice,R614
  10. Experimental Study of Influence of Atrovastatin on Expression of NF-κB in Retionpathy of Diabetic Rats,R587.1
  11. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3
  12. Study on Therapy and Mechanisms of the Role of Tang Kang in Diabetic Rat and Endothelial Cells,R285.5
  13. Effects of Hypothermia Resuscitation on PPAR-γ and Related Gene Expression in Rats Lung during Hemorrhagic Shock,R605.971
  14. The Research of Nf-kB、TNF-a and PLGF in the Pathogenesis and Severity of Preeclampsia and Theirs Relevance,R714.244
  15. Protein Expressions of EGFR、K-ras、P53 and Ki-67 in Colorectal Cancer, Gastric Cancer and Esophageal Cancer,R735
  16. Influence on NF-κB P65 and ASPP2 of Murine Lewis Lung Cancer of NF-кB Inhibitor PDTC and Cisplatin,R734.2
  17. Analysis of Efficacy of Radiotherapy and 5-FU Slow Release Particles in Treating Non-small Cell Lung Cancer,R734.2
  18. Restructuring Agkistrodon disintegrin Adinbitor human non-small cell lung cancer cell line A549,R285.5
  19. Sonic Hedgehog pathway and Caveolin-1 protein expression in esophageal cancer,R735.1
  20. NF-κB inhibitor PDTC on non-small cell lung cancer cells and JNK protein expression iASPP impact,R734.2
  21. Retinoic acid and matrine intervention rat hepatoma change process β-catenin, E-cadherin, MMP-7 dynamic changes,R735.7

CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Esophageal tumors
© 2012 www.DissertationTopic.Net  Mobile